Alterations of the Coxiella burnetii Replicative Vacuole Membrane Integrity and Interplay with the Autophagy Pathway

Front. Cell. Infect. Microbiol., 24 April 2017, https://doi.org/10.3389/fcimb.2017.00112 Coxiella burnetii, the etiologic agent of Q fever, is a Gram-negative obligate intracellular bacterium. It has been previously described that both the endocytic and autophagic pathways contribute to the Coxiella replicative vacuole (CRV) generation. Galectins are β-galactoside-binding lectins that accumulate in the cytosol before being secreted via En savoir plus surAlterations of the Coxiella burnetii Replicative Vacuole Membrane Integrity and Interplay with the Autophagy Pathway[…]

CLAFEM: correlative light atomic force electron microscopy

Methods in Cell Biology, http://doi.org.insb.bib.cnrs.fr/10.1016/bs.mcb.2017.03.010 Atomic force microscopy (AFM) is becoming increasingly used in the biology field. It can give highly accurate topography and biomechanical quantitative data, such as adhesion, elasticity, and viscosity, on living samples. Nowadays, correlative light electron microscopy is a must-have tool in the biology field that combines different microscopy techniques to spatially En savoir plus surCLAFEM: correlative light atomic force electron microscopy[…]

MET receptor variant R970C favors calpain-dependent generation of a fragment promoting epithelial cell scattering

Oncotarget. 2017 Jan 4. doi: 10.18632/oncotarget.14499 The receptor tyrosine kinase MET and its ligand, the hepatocyte growth factor, are essential to embryonic development, whereas deregulation of MET signaling is associated with tumorigenesis leading to various cancers, including lung carcinoma. Mutations in the MET kinase domain lead to constitutive kinase activity and are associated with tumorigenesis. En savoir plus surMET receptor variant R970C favors calpain-dependent generation of a fragment promoting epithelial cell scattering[…]

Characterization of a nuclear pore protein sheds light on the roles and composition of the Toxoplasma gondii nuclear pore complex

Cell Mol Life Sci. 2017 Jan 30. doi: 10.1007/s00018-017-2459-3. [Epub ahead of print] PMID: 28138739 The nuclear pore is a key structure in eukaryotes regulating nuclear-cytoplasmic transport as well as a wide range of cellular processes. Here, we report the characterization of the first Toxoplasma gondii nuclear pore protein, named TgNup302, which appears to be En savoir plus surCharacterization of a nuclear pore protein sheds light on the roles and composition of the Toxoplasma gondii nuclear pore complex[…]

Developmental Expression of 4-Repeat-Tau Induces Neuronal Aneuploidy in Drosophila Tauopathy Models

Sci Rep. 2017 Jan 23;7:40764. doi: 10.1038/srep40764. PMID: 28112163 Tau-mediated neurodegeneration in Alzheimer’s disease and tauopathies is generally assumed to start in a normally developed brain. However, several lines of evidence suggest that impaired Tau isoform expression during development could affect mitosis and ploidy in post-mitotic differentiated tissue. Interestingly, the relative expression levels of Tau En savoir plus surDevelopmental Expression of 4-Repeat-Tau Induces Neuronal Aneuploidy in Drosophila Tauopathy Models[…]

Adhesiveness of opportunistic Staphylococcus aureus to materials used in dental office: In vitro study

Microb Pathog. 2017 Feb;103:129-134. doi: 10.1016/j.micpath.2016.12.014. Epub 2016 Dec 18. PMID: 27993700 Staphylococcus aureus (S. aureus) is one of several opportunistic microbial pathogens associated with many healthcare problems. In the present study, S. aureus was assessed for its biofilm-forming ability on materials routinely used in dental offices, including stainless steel (SS), polyethylene (PE), and polyvinyl chloride En savoir plus surAdhesiveness of opportunistic Staphylococcus aureus to materials used in dental office: In vitro study[…]

Manipulation of host membranes by the bacterial pathogens Listeria, Francisella, Shigella and Yersinia

Semin Cell Dev Biol. 2016 Dec;60:155-167. doi: 10.1016/j.semcdb.2016.07.019. Epub 2016 Jul 19. Review. PMID: 27448494 Bacterial pathogens display an impressive arsenal of molecular mechanisms that allow survival in diverse host niches. Subversion of plasma membrane and cytoskeletal functions are common themes associated to infection by both extracellular and intracellular pathogens. Moreover, intracellular pathogens modify the En savoir plus surManipulation of host membranes by the bacterial pathogens Listeria, Francisella, Shigella and Yersinia[…]

ADAM30 Downregulates APP-Linked Defects Through Cathepsin D Activation in Alzheimer’s Disease

EBioMedicine. 2016 Jul;9:278-92. doi: 10.1016/j.ebiom.2016.06.002. Epub 2016 Jun 2. PMID: 27333034 Although several ADAMs (A disintegrin-like and metalloproteases) have been shown to contribute to the amyloid precursor protein (APP) metabolism, the full spectrum of metalloproteases involved in this metabolism remains to be established. Transcriptomic analyses centred on metalloprotease genes unraveled a 50% decrease in ADAM30 En savoir plus surADAM30 Downregulates APP-Linked Defects Through Cathepsin D Activation in Alzheimer’s Disease[…]

Unconventional endosome-like compartment and retromer complex in Toxoplasma gondii govern parasite integrity and host infection

Nat Commun. 2016 Apr 11;7:11191. doi: 10.1038/ncomms11191. PMID: 27064065 Membrane trafficking pathways play critical roles in Apicomplexa, a phylum of protozoan parasites that cause life-threatening diseases worldwide. Here we report the first retromer-trafficking interactome in Toxoplasma gondii. This retromer complex includes a trimer Vps35–Vps26–Vps29 core complex that serves as a hub for the endosome-like compartment and En savoir plus surUnconventional endosome-like compartment and retromer complex in Toxoplasma gondii govern parasite integrity and host infection[…]

TRANSAUTOPHAGY: European network for multidisciplinary research and translation of autophagy knowledge

Autophagy. 2016;12(3):614-7. doi: 10.1080/15548627.2016.1140294. PMID: 27046256 A collaborative consortium, named « TRANSAUTOPHAGY, » has been created among European research groups, comprising more than 150 scientists from 21 countries studying diverse branches of basic and translational autophagy. The consortium was approved in the framework of the Horizon 2020 Program in November 2015 as a COST Action of the En savoir plus surTRANSAUTOPHAGY: European network for multidisciplinary research and translation of autophagy knowledge[…]