Standardized Nanomechanical Atomic Force Microscopy Procedure (SNAP) for Measuring Soft and Biological Samples

Scientific Reports | 7: 5117 | DOI:10.1038/s41598-017-05383-0 We present a procedure that allows a reliable determination of the elastic (Young’s) modulus of soft samples, including living cells, by atomic force microscopy (AFM). The standardized nanomechanical AFM procedure (SNAP) ensures the precise adjustment of the AFM optical lever system, a prerequisite for all kinds of force En savoir plus surStandardized Nanomechanical Atomic Force Microscopy Procedure (SNAP) for Measuring Soft and Biological Samples[…]

Ezrin enhances line tension along transcellular tunnel edges via NMIIa driven actomyosin cable formation

NATURE COMMUNICATIONS | DOI: 10.1038/ncomms15839 Transendothelial cell macroaperture (TEM) tunnels control endothelium barrier function and are triggered by several toxins from pathogenic bacteria that provoke vascular leakage. Cellular dewetting theory predicted that a line tension of uncharacterized origin works at TEM boundaries to limit their widening. Here, by conducting high-resolution microscopy approaches we unveil the En savoir plus surEzrin enhances line tension along transcellular tunnel edges via NMIIa driven actomyosin cable formation[…]