ArfGAP1 restricts Mycobacterium tuberculosis entry by controlling the actin cytoskeleton

DOI 10.15252/embr.201744371| EMBO reports (2018) 19, 29-42 The interaction of Mycobacterium tuberculosis (Mtb) with pulmonary epithelial cells is critical for early stages of bacillus colonization and during the progression of tuberculosis. Entry of Mtb into epithelial cells has been shown to depend on F‐actin polymerization, though the molecular mechanisms are still unclear. Here, we demonstrate that En savoir plus surArfGAP1 restricts Mycobacterium tuberculosis entry by controlling the actin cytoskeleton[…]

Oligomannose-Rich Membranes of Dying Intestinal Epithelial Cells Promote Host Colonization by Adherent-Invasive E. coli

Front. Microbiol., 18 April 2018 | https://doi.org/10.3389/fmicb.2018.00742 A novel mechanism is revealed by which clinical isolates of adherent-invasive Escherichia coli (AIEC) penetrate into the epithelial cell layer, replicate, and establish biofilms in Crohn’s disease. AIEC uses the FimH fimbrial adhesin to bind to oligomannose glycans on the surface of host cells. Oligomannose glycans exposed on En savoir plus surOligomannose-Rich Membranes of Dying Intestinal Epithelial Cells Promote Host Colonization by Adherent-Invasive E. coli[…]

Biomechanical control of lysosomal secretion via the VAMP7 hub: a tug-of-war mechanism between V ARP and LRRK1

http://dx.doi.org/10.1101/159285 The rigidity of the cell environment can vary tremendously between tissues and in pathological conditions. How this property may affect intracellular membrane dynamics is still largely unknown. Here, using atomic force microscopy, we found that cells deficient in the secretory lysosome v-SNARE VAMP7 were impaired in adapting to substrate rigidity. Conversely VAMP7-mediated secretion was stimulated by more rigid En savoir plus surBiomechanical control of lysosomal secretion via the VAMP7 hub: a tug-of-war mechanism between V ARP and LRRK1[…]

Rv0613c/MSMEG_1285 Interacts with HBHA and Mediates Its Proper Cell-Surface Exposure in Mycobacteria

http://www.mdpi.com/1422-0067/19/6/1673 Heparin-binding haemagglutinin (HBHA) is a surface-exposed virulence factor of Mycobacterium tuberculosis and is involved in the binding of mycobacteria to non-phagocytic cells, allowing for extra-pulmonary dissemination of the bacilli. Despite its surface exposure, HBHA is not produced as a pre-protein containing a typical cleavable N-terminal signal peptide and is thus likely secreted by a En savoir plus surRv0613c/MSMEG_1285 Interacts with HBHA and Mediates Its Proper Cell-Surface Exposure in Mycobacteria[…]

Premature termination codon readthrough in human cells occurs in novel cytoplasmic foci and requires UPF proteins

http://jcs.biologists.org/content/130/18/3009.long Nonsense-mutation-containing messenger ribonucleoprotein particles (mRNPs) transit through cytoplasmic foci called P-bodies before undergoing nonsense-mediated mRNA decay (NMD), a cytoplasmic mRNA surveillance mechanism. This study shows that the cytoskeleton modulates transport of nonsense-mutation-containing mRNPs to and from P-bodies. Impairing the integrity of cytoskeleton causes inhibition of NMD. The cytoskeleton thus plays a crucial role in En savoir plus surPremature termination codon readthrough in human cells occurs in novel cytoplasmic foci and requires UPF proteins[…]

Role of Hydrophobins in Aspergillus fumigatus

http://www.mdpi.com/2309-608X/4/1/2 Resistance of Aspergillus fumigatus conidia to desiccation and their capacity to reach the alveoli are partly due to the presence of a hydrophobic layer composed of a protein from the hydrophobin family, called RodA, which covers the conidial surface. In A. fumigatus there are seven hydrophobins (RodA-RodG) belonging to class I and III. Most En savoir plus surRole of Hydrophobins in Aspergillus fumigatus[…]