Blocking bacterial entry at the adhesion step reveals dynamic recruitment of membrane and cytosolic probes

First published: 24 January 2019 https://doi.org/10.1111/boc.201800070 We report nanometric-scale, high-resolution, functional dynamic measurements of bacterial interaction with the host cell surface using photonic and adhesion force analyses. We describe how to achieve a precise monitoring of iterative cell–bacterium interactions to analyse host cell signalling responses to infection. By applying this method to Yersinia pseudotuberculosis, we En savoir plus surBlocking bacterial entry at the adhesion step reveals dynamic recruitment of membrane and cytosolic probes[…]

Stiffness tomography of eukaryotic intracellular compartments by atomic force microscopy

DOI 10.1039/C8NR08955H Precise localization and biophysical characterization of cellular structures is a key to the understanding of biological processes happening both inside the cell and at the cell surface. Atomic force microscopy is a powerful tool to study the cell surface – topography, elasticity, viscosity, interactions – and also the visco- elastic behavior of the En savoir plus surStiffness tomography of eukaryotic intracellular compartments by atomic force microscopy[…]

Transversal elasticity of TIEG1 KO muscle fibers probed by atomic force microscopy

Inhibition of TIEG1 gene provides changes within the musculoskeletal system [1-2] such as hyperplasia, hypertrophy and a metabolic glycolitic switch for slow and fast fibers [3]. Previously, mechanical tests have been performed on single muscle fibers using stretch and relaxation tests in the direction of the main fiber filaments. The functional properties of TIEG1 KO En savoir plus surTransversal elasticity of TIEG1 KO muscle fibers probed by atomic force microscopy[…]

Development of a novel multiphysical approach for the characterization of mechanical properties of musculotendinous tissues

Scientific Reportsvolume 9, Article number: 7733 (2019) At present, there is a lack of well-validated protocols that allow for the analysis of the mechanical properties of muscle and tendon tissues. Further, there are no reports regarding characterization of mouse skeletal muscle and tendon mechanical properties in vivo using elastography thereby limiting the ability to monitor En savoir plus surDevelopment of a novel multiphysical approach for the characterization of mechanical properties of musculotendinous tissues[…]

Automated multi-sample acquisition and analysis using atomic force microscopy for biomedical applications

https://doi-org.insb.bib.cnrs.fr/10.1371/journal.pone.0213853 In the last 20 years, atomic force microscopy (AFM) has emerged as a ubiquitous technique in biological research, allowing the analysis of biological samples under near-physiological conditions from single molecules to living cells. Despite its growing use, the low process throughput remains a major drawback. Here, we propose a solution validated on a device En savoir plus surAutomated multi-sample acquisition and analysis using atomic force microscopy for biomedical applications[…]

10 ans des Apprench’tis Chercheurs

L’Arbre des Connaissances vous donne rendez-vous du 15 mai au 12 juin 2019 pour la 15e saison des Congrès des Apprentis Chercheurs. Tout au long de l’année scolaire près de 400 collégiens et lycéens de métropole et outre-mer ont découvert l’univers et les méthodes de la recherche grâce à leur participation au dispositif Apprentis Chercheurs. En savoir plus sur10 ans des Apprench’tis Chercheurs[…]

MET amplification increases the metastatic spread of EGFR-mutated NSCLC

Published online: September 11, 2018 – DOI: https://doi.org/10.1016/j.lungcan.2018.09.008 Background Five to 20% of metastatic EGFR-mutated non-small cell lung cancers (NSCLC) develop acquired resistance to EGFR tyrosine kinase inhibitors (EGFR-TKI) through MET amplification. The effects of MET amplification on tumor and patient phenotype remain unknown. Methods We investigated,in vitro and in vivo, the impact of MET En savoir plus surMET amplification increases the metastatic spread of EGFR-mutated NSCLC[…]

MAGP-1 and fibronectin control EGFL7 functions by driving its deposition into distinct endothelial extracellular matrix locations

19 October 2018 – https://doi.org/10.1111/febs.14680 The extracellular matrix (ECM) is essential to provide mechanical support to tissues but is also a bioactive edifice which controls cell behavior. Cell signaling generated by ECM components through integrin-mediated contacts, modulates cell biological activity. In addition, by sequestrating or releasing growth factors, the ECM is an active player of physiological En savoir plus surMAGP-1 and fibronectin control EGFL7 functions by driving its deposition into distinct endothelial extracellular matrix locations[…]

Fifteen years of Servitude et Grandeur to the application of a biophysical technique in medicine: The tale of AFMBioMed

 18 December 2018 – https://doi.org/10.1002/jmr.2773 AFMBioMed is the founding name under which international conferences and summer schools are organized around the application of atomic force microscopy in life sciences and nanomedicine. From its inception at the Atomic Energy Commission in Marcoule near 2004 to its creation in 2007 and to its 10th anniversary conference in Krakow, En savoir plus surFifteen years of Servitude et Grandeur to the application of a biophysical technique in medicine: The tale of AFMBioMed[…]