First published: 24 January 2019 https://doi.org/10.1111/boc.201800070 We report nanometric-scale, high-resolution, functional dynamic measurements of bacterial interaction with the host cell surface using photonic and adhesion force analyses. We describe how to achieve a precise monitoring of iterative cell–bacterium interactions to analyse host cell signalling responses to infection. By applying this method to Yersinia pseudotuberculosis, we En savoir plus surBlocking bacterial entry at the adhesion step reveals dynamic recruitment of membrane and cytosolic probes[…]

DOI 10.1039/C8NR08955H Precise localization and biophysical characterization of cellular structures is a key to the understanding of biological processes happening both inside the cell and at the cell surface. Atomic force microscopy is a powerful tool to study the cell surface – topography, elasticity, viscosity, interactions – and also the visco- elastic behavior of the En savoir plus surStiffness tomography of eukaryotic intracellular compartments by atomic force microscopy[…]

Inhibition of TIEG1 gene provides changes within the musculoskeletal system [1-2] such as hyperplasia, hypertrophy and a metabolic glycolitic switch for slow and fast fibers [3]. Previously, mechanical tests have been performed on single muscle fibers using stretch and relaxation tests in the direction of the main fiber filaments. The functional properties of TIEG1 KO En savoir plus surTransversal elasticity of TIEG1 KO muscle fibers probed by atomic force microscopy[…]

Scientific Reportsvolume 9, Article number: 7733 (2019) At present, there is a lack of well-validated protocols that allow for the analysis of the mechanical properties of muscle and tendon tissues. Further, there are no reports regarding characterization of mouse skeletal muscle and tendon mechanical properties in vivo using elastography thereby limiting the ability to monitor En savoir plus surDevelopment of a novel multiphysical approach for the characterization of mechanical properties of musculotendinous tissues[…]

https://doi-org.insb.bib.cnrs.fr/10.1371/journal.pone.0213853 In the last 20 years, atomic force microscopy (AFM) has emerged as a ubiquitous technique in biological research, allowing the analysis of biological samples under near-physiological conditions from single molecules to living cells. Despite its growing use, the low process throughput remains a major drawback. Here, we propose a solution validated on a device En savoir plus surAutomated multi-sample acquisition and analysis using atomic force microscopy for biomedical applications[…]