Adherent-Invasive and Non-Invasive Escherichia coli Isolates Differ in Their Effects on Caenorhabditis elegans’ Lifespan

Microorganisms 2021, 9(9), 1823 Le pathotype Escherichia coli adhérent-invasif (AIEC) a été impliqué dans la pathogenèse des maladies inflammatoires de l’intestin en général et dans la maladie de Crohn (MC) en particulier. Les souches AIEC sont principalement caractérisées par leur capacité à adhérer aux cellules épithéliales intestinales et à les envahir. Cependant, les caractéristiques génétiques En savoir plus surAdherent-Invasive and Non-Invasive Escherichia coli Isolates Differ in Their Effects on Caenorhabditis elegans’ Lifespan[…]

Persistence and dynamics of fluorescent Lactobacillus plantarum in the healthy versus inflamed gut

Gut Microbes, Published online: 28 Mar 2021 The gastrointestinal tract is the main ecological niche in which Lactobacillus strains may provide health benefits in mammals. There is currently a need to characterize host-microbe interactions in space and time by tracking these bacteria in vivo. We combined noninvasive whole-body imaging with ex vivo fluorescence confocal microscopy En savoir plus surPersistence and dynamics of fluorescent Lactobacillus plantarum in the healthy versus inflamed gut[…]

Assessment of Pb(II), Cd(II), and Al(III) Removal Capacity of Bacteria from Food and Gut Ecological Niches: Insights into Biodiversity to Limit Intestinal Biodisponibility of Toxic Metals

Published: 22 February 2021, Microorganisms Toxic metals (such as lead, cadmium, and, to a lesser extent, aluminum) are detrimental to health when ingested in food or water or when inhaled. By interacting with heavy metals, gut and food-derived microbes can actively and/or passively modulate (by adsorption and/or seques- tration) the bioavailability of these toxins inside En savoir plus surAssessment of Pb(II), Cd(II), and Al(III) Removal Capacity of Bacteria from Food and Gut Ecological Niches: Insights into Biodiversity to Limit Intestinal Biodisponibility of Toxic Metals[…]

Correlative Microscopy Using Scanning Probe Microscopes

First published: 06 September 2019 https://doi.org/10.1002/9781119086420.ch6 Scanning probe microscopy methods have achieved nanoscopic views with unprecedented resolution, spanning scales from the atomic all the way to the tissue level. The scanning probe microscopy approach has been extended from scanning tunneling microscope (STM) to a large number of imaging modalities, such as atomic force microscopy (AFM), En savoir plus surCorrelative Microscopy Using Scanning Probe Microscopes[…]

2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations

Published: 20 March 2020 https://doi.org/10.1038/s41467-020-15140-z Nonsense mutations cause about 10% of genetic disease cases, and no treatments are available. Nonsense mutations can be corrected by molecules with nonsense mutation readthrough activity. An extract of the mushroom Lepista inversa has recently shown high-efficiency correction of UGA and UAA nonsense mutations. One active constituent of this extract En savoir plus sur2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations[…]

Rab11A regulates dense granule transport and secretion during Toxoplasma gondii invasion of host cells and parasite replication

Published: May 28, 2020 https://doi.org/10.1371/journal.ppat.1008106 Toxoplasma gondii possesses an armada of secreted virulent factors that enable parasite invasion and survival into host cells. These factors are contained in specific secretory organelles, the rhoptries, micronemes and dense granules that release their content upon host cell recognition. Dense granules are secreted in a constitutive manner during parasite En savoir plus surRab11A regulates dense granule transport and secretion during Toxoplasma gondii invasion of host cells and parasite replication[…]

Galectin-3 modulates epithelial cell adaptation to stress at the ER-mitochondria interface

Published: 12 May 2020 https://doi.org/10.1038/s41419-020-2556-3 Cellular stress response contributes to epithelial defense in adaptation to environment changes. Galectins play a pivotal role in the regulation of this response in malignant cells. However, precise underlying mechanisms are largely unknown. Here we demonstrate that Galectin-3, a pro and anti-apoptotic lectin, is required for setting up a correct En savoir plus surGalectin-3 modulates epithelial cell adaptation to stress at the ER-mitochondria interface[…]

High‐dose dietary supplementation with zinc prevents gut inflammation: Investigation of the role of metallothioneins and beyond by transcriptomic and metagenomic studies

First published: 30 July 2020 https://doi.org/10.1096/fj.202000562RR Although it is known that zinc has several beneficial roles in the context of gut inflammation, the underlying mechanisms have not been extensively characterized. Zinc (Zn) is known to be the primary physiological inducer of the expression of the metallothionein (MT) superfamily of small stress‐responsive proteins. The expression of En savoir plus surHigh‐dose dietary supplementation with zinc prevents gut inflammation: Investigation of the role of metallothioneins and beyond by transcriptomic and metagenomic studies[…]

Blocking bacterial entry at the adhesion step reveals dynamic recruitment of membrane and cytosolic probes

First published: 24 January 2019 https://doi.org/10.1111/boc.201800070 We report nanometric-scale, high-resolution, functional dynamic measurements of bacterial interaction with the host cell surface using photonic and adhesion force analyses. We describe how to achieve a precise monitoring of iterative cell–bacterium interactions to analyse host cell signalling responses to infection. By applying this method to Yersinia pseudotuberculosis, we En savoir plus surBlocking bacterial entry at the adhesion step reveals dynamic recruitment of membrane and cytosolic probes[…]

Stiffness tomography of eukaryotic intracellular compartments by atomic force microscopy

DOI 10.1039/C8NR08955H Precise localization and biophysical characterization of cellular structures is a key to the understanding of biological processes happening both inside the cell and at the cell surface. Atomic force microscopy is a powerful tool to study the cell surface – topography, elasticity, viscosity, interactions – and also the visco- elastic behavior of the En savoir plus surStiffness tomography of eukaryotic intracellular compartments by atomic force microscopy[…]