Correlative Microscopy Using Scanning Probe Microscopes

First published: 06 September 2019

Scanning probe microscopy methods have achieved nanoscopic views with unprecedented resolution, spanning scales from the atomic all the way to the tissue level. The scanning probe microscopy approach has been extended from scanning tunneling microscope (STM) to a large number of imaging modalities, such as atomic force microscopy (AFM), Kelvin probe force microscopy (KFM), scanning near‐field optical microscopy (SNOM), magnetic force microscopy (MFM), scanning tunnelling potentiometry (STP), and spinpolarized scanning tunnelling microscopy (SPSTM). These techniques offer the possibility to observe atoms at unprecedented scale and even to manipulate them. Hence, in the context of correlative microscopy related to AFM and applied in life sciences, one AFM offers correlation either with topograms or with diverse biophysics maps. In 2001, high‐speed (HS)‐AFM was released with the visualization at unpreceded resolution and dynamics of the walking‐like movement of machine‐at‐work as molecular motors on cytoskeleton filaments.

Georg Fantner & Frank Lafont

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