DHA-phospholipids control membrane fusion and transcellular tunnel dynamics

J Cell Sci jcs.259119. | 08 DECEMBER 2021 Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (PLs) in physiology. Here, we investigated the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) triggered by RhoA inhibition-associated cell spreading. Lipidomic analyses show that human Read more about DHA-phospholipids control membrane fusion and transcellular tunnel dynamics[…]

Interactions of hydrophilic quantum dots with defect-free and defect containing supported lipid membranes

Colloids and Surfaces B: Biointerfaces Volume 210, February 2022, 112239 Quantum dots (QDs) are semiconductor nanoparticles with unique optical and electronic properties, whose interest as potential nano-theranostic platforms for imaging and sensing is increasing. The design and use of QDs requires the understanding of cell-nanoparticle interactions at a microscopic and nanoscale level. Model systems such Read more about Interactions of hydrophilic quantum dots with defect-free and defect containing supported lipid membranes[…]

Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation

Nature Physics (2021) The early development of many organisms involves the folding of cell monolayers, but this behaviour is difficult to reproduce in vitro; therefore, both mechanistic causes and effects of local curvature remain unclear. Here we study epithelial cell monolayers on corrugated hydrogels engineered into wavy patterns, examining how concave and convex curvatures affect Read more about Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation[…]

Adherent-Invasive and Non-Invasive Escherichia coli Isolates Differ in Their Effects on Caenorhabditis elegans’ Lifespan

Microorganisms 2021, 9(9), 1823 The adherent-invasive Escherichia coli (AIEC) pathotype has been implicated in the pathogenesis of inflammatory bowel diseases in general and in Crohn’s disease (CD) in particular. AIEC strains are primarily characterized by their ability to adhere to and invade intestinal epithelial cells. However, the genetic and phenotypic features of AIEC isolates vary Read more about Adherent-Invasive and Non-Invasive Escherichia coli Isolates Differ in Their Effects on Caenorhabditis elegans’ Lifespan[…]

Persistence and dynamics of fluorescent Lactobacillus plantarum in the healthy versus inflamed gut

Gut Microbes, Published online: 28 Mar 2021 The gastrointestinal tract is the main ecological niche in which Lactobacillus strains may provide health benefits in mammals. There is currently a need to characterize host-microbe interactions in space and time by tracking these bacteria in vivo. We combined noninvasive whole-body imaging with ex vivo fluorescence confocal microscopy Read more about Persistence and dynamics of fluorescent Lactobacillus plantarum in the healthy versus inflamed gut[…]

Assessment of Pb(II), Cd(II), and Al(III) Removal Capacity of Bacteria from Food and Gut Ecological Niches: Insights into Biodiversity to Limit Intestinal Biodisponibility of Toxic Metals

Published: 22 February 2021, Microorganisms Toxic metals (such as lead, cadmium, and, to a lesser extent, aluminum) are detrimental to health when ingested in food or water or when inhaled. By interacting with heavy metals, gut and food-derived microbes can actively and/or passively modulate (by adsorption and/or seques- tration) the bioavailability of these toxins inside Read more about Assessment of Pb(II), Cd(II), and Al(III) Removal Capacity of Bacteria from Food and Gut Ecological Niches: Insights into Biodiversity to Limit Intestinal Biodisponibility of Toxic Metals[…]

Correlative Microscopy Using Scanning Probe Microscopes

First published: 06 September 2019 https://doi.org/10.1002/9781119086420.ch6 Scanning probe microscopy methods have achieved nanoscopic views with unprecedented resolution, spanning scales from the atomic all the way to the tissue level. The scanning probe microscopy approach has been extended from scanning tunneling microscope (STM) to a large number of imaging modalities, such as atomic force microscopy (AFM), Read more about Correlative Microscopy Using Scanning Probe Microscopes[…]

2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations

Published: 20 March 2020 https://doi.org/10.1038/s41467-020-15140-z Nonsense mutations cause about 10% of genetic disease cases, and no treatments are available. Nonsense mutations can be corrected by molecules with nonsense mutation readthrough activity. An extract of the mushroom Lepista inversa has recently shown high-efficiency correction of UGA and UAA nonsense mutations. One active constituent of this extract Read more about 2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations[…]

Rab11A regulates dense granule transport and secretion during Toxoplasma gondii invasion of host cells and parasite replication

Published: May 28, 2020 https://doi.org/10.1371/journal.ppat.1008106 Toxoplasma gondii possesses an armada of secreted virulent factors that enable parasite invasion and survival into host cells. These factors are contained in specific secretory organelles, the rhoptries, micronemes and dense granules that release their content upon host cell recognition. Dense granules are secreted in a constitutive manner during parasite Read more about Rab11A regulates dense granule transport and secretion during Toxoplasma gondii invasion of host cells and parasite replication[…]

Galectin-3 modulates epithelial cell adaptation to stress at the ER-mitochondria interface

Published: 12 May 2020 https://doi.org/10.1038/s41419-020-2556-3 Cellular stress response contributes to epithelial defense in adaptation to environment changes. Galectins play a pivotal role in the regulation of this response in malignant cells. However, precise underlying mechanisms are largely unknown. Here we demonstrate that Galectin-3, a pro and anti-apoptotic lectin, is required for setting up a correct Read more about Galectin-3 modulates epithelial cell adaptation to stress at the ER-mitochondria interface[…]