4 May 2016

Cytoadhesion of Plasmodium falciparum-infected erythrocytes, ultrastructural characterization and elastic properties of knobs studied with AFM

In collaboration with D. Mattei, A. Scherf, CNRS-URA2581, Institut Pasteur, Paris

Using Atomic Force Microscopy (AFM), we characterize the structure and the elastic properties of the knobs induced at the plasma membrane of red blood cells, which are infected with Plasmodium falciparum, the causative agent of Malaria. By comparing wild type and mutant strains, we examine whether a link exists between genotype and phenotype based on the knob-dependent cytoadhesion. Using AFM tips functionalized with ligands of parasite-induced cell surface expressed proteins, we analyze how the mechanical features of the knobs influence the cytoadhesion.

Sébastien Janel, Sébastien Pomel

Financial support : French Minister for Research, National Agency for Research (Microbiology 2005), Medical Research Foundation, European community and Regional Council

-Nacer, A, E Roux, S Pomel, C Scheideig-Benatar, H Sakamoto, F. Lafont, A. Scherf and D. Matei. Surface Exposure of Non-functional PfEMP1 in Plasmodium falciparum with Chromosome 9 Deletion is Not Linked to clag9 Expression PLoS ONE 2011 6(12) :e29039