Microb Pathog. 2016 Feb;91:61-7. doi: 10.1016/j.micpath.2015.11.009. Epub 2015 Nov 24.
Orthodontic and other oral appliances act as reservoir of opportunistic pathogens that can easily become resistant to antibiotics and cause systemic infections. The aim of this study was to investigate the ability of Staphylococcus aureus strains isolated from healthy patients with orthodontic appliances, to adhere to biotic (HeLa cells) and abiotic surfaces (polystyrene and dental alloy). Adhesive ability to polystyrenewas tested by crystal violet staining and quantitative biofilm production on dental alloy surfaces was eval- uated by MTT reduction assay. In addition, the presence of icaA and icaD genes was achieved by poly- merase chain reaction (PCR). Qualitative biofilm production revealed that 70.6% of strains were slime producers. The metabolic activity of S. aureus biofilms on dental alloy surfaceswas high and did not differ between tested strains. Moreover, all the isolates were adhesive to HeLa cells and 94% of them harbor icaA and icaD genes. Considerable adhesion and internalization capacity to the epithelial HeLa cells and strong biofilm production abilities together, with a high genotypic expression of icaA/icaD genes are an important equipment of S. aureus to colonize orthodontic appliances and eventually to disseminate to- wards other body areas.
Merghni A, Ben Nejma M, Dallel I, Tobji S, Ben Amor A, Janel S, Lafont F, Aouni M, Mastouri M.