Sébastien Janel

Ingénieur de Recherche CN CNRS

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Atomic force microscopy applied to microbiology: topography, adhesion & mechanical properties of biological samples. In charge of the AFM facility in Institut Pasteur de Lille (6 instruments).
Teaching students, promoting the AFM for bio labs and the general public.

→ Atomic Force Microscopy in the lab

Some projects:

EDUCATION

2003: DESS Télédétection & Imagerie numérique, Centre d’Etudes Spatiales et des Rayonnements, Toulouse, France.
2002: BSc of Science (Honours), 2.1, Applied Physics, Nottingham Trent University, UK.
2000: DUT Mesures Physiques, Institut Universitaire de Technologie, Limoges, France.
1998: Baccalauréat ScientifiqueLycée Paul Eluard, Saint-Junien, France.

EXPERIENCE

2018-: Ingénieur de Recherche, CNRS UMR9017, Lille, France
2009-2017: Ingénieur d’Études CNRS UMR8204, Lille, France
2007-09: Ingénieur d’Études, Institut pasteur Lille, France
2004-06: Ingénieur d’Études, Biointerfaces, IRI @ IEMN, Lille, France

TEACHING

AFM Biomed Summerschool 2009-2019.
Master de Biophysique, Université de Lille.
D.U. Microbiologie Cellulaire – Institut Pasteur de Lille 07/2009-2011.
EMBO Cell Biology of Host-Pathogens Interactions – Institut Pasteur Paris 08/2008.
Formation Microscopies à Champ Proche – Institut Pasteur de Lille 03/2008.
Apprentis chercheurs, Kid Campus, Maison pour la science, Fête de la science.

PUBLICATIONS

  1. Janel, S., Popoff, M., Barois, N., Werkmeister, E., Divoux, S., Perez, F., & Lafont, F. (2019). Stiffness tomography of eukaryotic intracellular compartments by atomic force microscopy. Nanoscale.
  2. Soyer, M., Ciczora, Y., Lafont, F., Popoff, M., Werkmeister, E., & Janel, S. (2019). Blocking bacterial entry at the adhesion step reveals dynamic recruitment of membrane and cytosolic probes. Biology of the Cell, 1–11.
  3. Stefani, C., Gonzalez-Rodriguez, D., Senju, Y., Doye, A., Efimova, N., Janel, S., … Lemichez, E. (2017). Ezrin enhances line tension along transcellular tunnel edges via NMIIa driven actomyosin cable formation. Nature Communications, 8, 15839.
  4. Maïssa, N., Covarelli, V., Janel, S., Durel, B., Simpson, N., Bernard, S. C., … Bourdoulous, S. (2017). Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β 2-Adrenoceptor clusters assembled by alpha-Actinin-4. Nature Communications, 8, 15764.
  5. Merghni, A., Bekir, K., Kadmi, Y., Dallel, I., Janel, S., Bovio, S., … Mastouri, M. (2017). Adhesiveness of opportunistic Staphylococcus aureus to materials used in dental office: In vitro study. Microbial Pathogenesis, 103, 129–134.
  6. Janel, S., Werkmeister, E., Bongiovanni, A., Lafont, F., & Barois, N. (2017). CLAFEM: Correlative light atomic force electron microscopy. Methods in Cell Biology, 140, 165–185.
  7. Merghni, A., Kammoun, D., Hentati, H., Janel, S., Popoff, M., Lafont, F., … Mastouri, M. (2016). Quantification of Staphylococcus aureus adhesion forces on various dental restorative materials using atomic force microscopy. Applied Surface Science, 379, 323–330.
  8. Merghni, A., Ben Nejma, M., Dallel, I., Tobji, S., Ben Amor, A., Janel, S., … Mastouri, M. (2016). High potential of adhesion to biotic and abiotic surfaces by opportunistic Staphylococcus aureus strains isolated from orthodontic appliances. Microbial Pathogenesis, 91, 61–67.
  9. Rodriguez-Emmenegger, C., Janel, S., de los Santos Pereira, A., Bruns, M., & Lafont, F. (2015). Quantifying bacterial adhesion on antifouling polymer brushes via single-cell force spectroscopy. Polym. Chem., 6(31), 5740–5751.
  10. Bernard, S. C., Simpson, N., Join-Lambert, O., Federici, C., Laran-Chich, M. P., Maïssa, N., … Bourdoulous, S. (2014). Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization. Nature Medicine, 20(7), 725–731.
  11. Boulbene, B., Morchain, J., Bonin, M. M., Janel, S., Lafont, F., & Schmitz, P. (2012). A combined computational fluid dynamics (CFD) and experimental approach to quantify the adhesion force of bacterial cells attached to a plane surface. AIChE Journal, 58(12), 3614–3624.
  12. Gonzalez-Rodriguez, D., Maddugoda, M. P., Stefani, C., Janel, S., Lafont, F., Cuvelier, D., … Brochard-Wyart, F. (2012). Cellular dewetting: Opening of macroapertures in endothelial cells. Physical Review Letters, 108(21), 218105.
  13. Mostowy, S., Janel, S., Forestier, C., Roduit, C., Kasas, S., Pizarro-Cerdá, J., … Lafont, F. (2011). A role for septins in the interaction between the Listeria monocytogenes invasion protein InlB and the Met receptor. Biophysical Journal, 100(8), 1949–1959.
  14. Maddugoda, M. P., Stefani, C., Gonzalez-Rodriguez, D., Saarikangas, J., Torrino, S., Janel, S., … Lemichez, E. (2011). CAMP signaling by Anthrax edema toxin induces transendothelial cell tunnels, which Are resealed by MIM via Arp2/3-Driven actin polymerization. Cell Host and Microbe, 10(5), 464–474.
  15. Coffinier, Y., Janel, S., Addad, A., Blossey, R., Gengembre, L., Payen, E., & Boukherroub, R. (2007). Preparation of superhydrophobic silicon oxide nanowire surfaces. Langmuir, 23(4), 1608–1611.
  16. Szunerits, S., Mermoux, M., Crisci, A., Marcus, B., Bouvier, P., Delabouglise, D., … Tay, L. (2006). Raman imaging and Kelvin probe microscopy for the examination of the heterogeneity of doping in polycrystalline boron-doped diamond electrodes. Journal of Physical Chemistry B, 110(47), 23888–23897.
  17. Szunerits, S., Coffinier, Y., Janel, S., & Boukherroub, R. (2006). Stability of the gold/silica thin film interface: Electrochemical and surface plasmon resonance studies. Langmuir, 22(25), 10716–10722.
  18. Rousselet-Perraut, K., Chesneau, O., Vakili, F., Mourard, D., Janel, S., Lavaud, L., & Crocherie, A. (2003). Resolving polarized stellar features thanks to polarimetric interferometry. In S. Fineschi (Ed.), Astronomical Telescopes and Instrumentation (pp. 448–455). International Society for Optics and Photonics.

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